Voltage dependence of K+ channels in guard-cell protoplasts

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Voltage dependence of K channels in guard-cell protoplasts.

Stomatal pores in leaves enable plants to regulate the exchange of gases with their environment. Variations of the pore aperture are mediated by controlled changes of potassium salt concentrations in the surrounding guard cells. The voltage-dependent gating of K(+)-selective channels in the plasma membrane (plasmalemma) of cell-wall-free guard cells (protoplasts) was studied at the molecular le...

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External pH effects on the depolarization-activated K channels in guard cell protoplasts of Vicia faba

Previous studies reveal that the pH of the apoplastic solution in the guard cell walls may vary between 7.2 and 5.1 in closed and open stomata, respectively. During these aperture and pH changes, massive K+ fluxes cross the cellular plasma membrane driving the osmotic turgor and volume changes of guard cells. Therefore, we examined the effect of extracellular pH on the depolarization-activated ...

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The number of K(+) channels in the plasma membrane of guard cell protoplasts changes in parallel with the surface area.

The activity of the two dominant K(+) channels in the plasma membrane of Vicia faba guard cell protoplasts was examined during pressure-driven swelling. For this purpose, the K(+) currents and the membrane capacitance (C(m)) of guard cell protoplasts were recorded in parallel. A rise in C(m), reflecting an increase of the membrane surface area, was coupled to a proportional rise in conductance ...

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Slo3 K+ Channels: Voltage and pH Dependence of Macroscopic Currents

The mouse Slo3 gene (KCNMA3) encodes a K(+) channel that is regulated by changes in cytosolic pH. Like Slo1 subunits responsible for the Ca(2+) and voltage-activated BK-type channel, the Slo3 alpha subunit contains a pore module with homology to voltage-gated K(+) channels and also an extensive cytosolic C terminus thought to be responsible for ligand dependence. For the Slo3 K(+) channel, incr...

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AKT2/3 Subunits Render Guard Cell K+ Channels Ca2+ Sensitive

Inward-rectifying K+ channels serve as a major pathway for Ca2+-sensitive K+ influx into guard cells. Arabidopsis thaliana guard cell inward-rectifying K+ channels are assembled from multiple K+ channel subunits. Following the recent isolation and characterization of an akt2/3-1 knockout mutant, we examined whether the AKT2/3 subunit carries the Ca2+ sensitivity of the guard cell inward rectifi...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1987

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.84.12.4108